Table 1 Strains and plasmids used in this study

Bacteria

E. piscicida

PPD130/91

Wild type, Km^s, Col^r, Amp^s, LD₅₀ = 10^5.2

⁵⁴

^ΔeseJ

in-frame deletion 1–1359 aa of EseJ, PPD130/91

¹⁷

^ΔeseJ[eseJ]

ΔeseJ with pACYC-eseJ-HA, PPD130/91

¹⁷

^WT/GFP

PPD130/91 with pFPV25.1

⁴⁴

^ΔeseJ/GFP

ΔeseJ with pFPV25.1

¹⁷

^{ΔeseJ[eseJ]/GFP}

ΔeseJ[eseJ] with pFPV25.1

¹¹

^ΔfimA

in-frame deletion 1–178 aa of FimA, PPD130/91

¹¹

^ΔfimH

in-frame deletion 1–357 aa of FimH, PPD130/91

¹¹

^ΔeseJΔfimA

in-frame deletion of EseJ and FimA, PPD130/91

¹¹

^ΔeseJΔfimH

in-frame deletion of EseJ and FimH, PPD130/91

¹¹

^ΔfimH[eseJ]

ΔfimH with pACYC-eseJ-HA, PPD130/91

This study

E. coli

DH5α

α complementation

Stratagene

Plasmids

pFPV25.1

Derivative of pBR322, gfpmut3A under constitutive promoter

⁵⁵

pACYC-eseJ -HA

pACYC184 with eseJ-HA, Tet^r

¹⁷

pLVX-caspase-8 siRNA

pLVX-shRNA2-Puro with RNAi sequence of caspase-8, Amp^r

This study

pLVX-nlrp3 siRNA

pLVX-shRNA2-Puro with RNAi sequence of nlrp3, Amp^r

This study

pLVX-control siRNA

pLVX-shRNA2-Puro with scrambled sequence, Amp^r

This study

pMD2.g

Lentiviral envelope vector, Amp^r

⁵⁶

pSPAX2

Lentiviral packaging vector, Amp^r

⁵⁶

pLVX-shRNA2-Puro

Lentiviral expression vector expressing a small hairpin RNA for RNA interference, Amp^r

Fenghuibio