Fig. 3: Effect of high levels of β-OHB on memory and hippocampal CaMKII-α kbhb. | Communications Biology

Fig. 3: Effect of high levels of β-OHB on memory and hippocampal CaMKII-α kbhb.

From: Hippocampal CaMKII-α β-hydroxybutyrylation induces memory deficits in mice with type 1 diabetes mellitus

Fig. 3

A, B Blood and hippocampal levels of β-OHB in mice injected with β-OHB or saline for 10 days (blood β-OHB: n = 6 per group; hippocampal β-OHB: n = 6 per group). C–E Mice were injected with β-OHB or saline, and the Y-maze test, novel object location test and novel object recognition test were subsequently performed (Y-maze test: n = 7 per group; novel object position test: n = 7 per group; novel object recognition test: n = 8 per group). F–H Freezing behavior in context A (conditioned) and context B (unconditioned) and the discrimination index of mice injected with β-OHB or saline for 10 days (n = 7 per group). I Hippocampal HDAC activity in mice injected with β-OHB or saline for 10 days (n = 6 per group). J Hippocampal H2Aac, H2A, H3ac, H3, H4ac and H4 levels in mice injected with β-OHB or saline for 10 days (n = 6 per group). K, L Degree of hippocampal CaMKII-α kbhb at the K42 and K267 sites and CaMKII activity in mice injected with β-OHB or saline for 10 days (n = 6 per group). The data are presented as the means ± SEMs; unpaired t test; *p < 0.05, ***p < 0.001 and ****p < 0.0001. bhb: β-hydroxybutyrylation.

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