Fig. 1: The basic process of ABMP approach and activity-based probes of AGALs.

a Workflow of the ABMP approach for high-throughput screening of gut microbiome samples. b The AGAL activity-based probe (ABP1 and ABP2) structure with a cyclophellitol-aziridine scaffold for selective labeling of active AGALs. c Mechanism-based inhibition of active AGALs by cyclophellitol-derived ABPs.