Fig. 7: Hydrolysis and transglycosylation activity of AGALs towards natural sugars analyzed by TLC and LC-MS. | Communications Chemistry

Fig. 7: Hydrolysis and transglycosylation activity of AGALs towards natural sugars analyzed by TLC and LC-MS.

From: Activity-based metaproteomics driven discovery and enzymological characterization of potential α-galactosidases in the mouse gut microbiome

Fig. 7

a Reaction process and TLC analysis of stachyose hydrolysis by AGAL1. b Reaction process and TLC analysis of blood group antigen trisaccharide hydrolysis by AGAL3. c LC-MS SCAN and SRM analysis of blood group B antigen trisaccharide: Blood group B trisaccharide, [M + H]+ = 489.3, [M+Na]+=511.2, [M + K]+ = 533.1; Galactose, [M + 2PMP-H2O + H]+ = 511.3, [M + 2PMP-H2O + Na]+=533.2, [M + 2PMP-H2O + K]+ = 549.1; Disaccharide, [M + 2PMP-H2O + H]+ = 657.3, [M + 2PMP-H2O + Na]+=679.3, [M + 2PMP-H2O + K]+ = 695.2. d Reaction process and TLC analysis of transglycosylation towards monosaccharides and polysaccharides by AGAL6.

Back to article page