Table 2 Characterization of the genes belonging to knm BGC and comparison with their orthologues from bal (balhimycin BGC from Am. balhimycina DSM 5908) and ris (ristocetin BGC from Amycolatopsis sp. TNS 106)
knm | bal (nucleic acid sequence identity) | ris (nucleic acid sequence identity) | Product (function) |
|---|---|---|---|
knm1 | a | ORF1 (78%) | VanH (GPA resistance d-Lactate dehydrogenase) |
knm2 | a | ORF2 (81,5%) | VanA (GPA resistance d-Ala-d-Lac ligase) |
knm3 | a | ORF3 (82%) | VanX (GPA resistance d,d-dipeptidase) |
knm4 | vnlSAb (86%) | a | VanS (GPA resistance two-component regulatory system sensor histidine kinase) |
knm5 | vnlRAb (85%) | a | VanR (GPA two-component regulatory system sensor resistance response regulator) |
knm6 | vanYAb (77%) | a | VanY (GPA resistance d,d-carboxypeptidase) |
knm7R | bbr (85%) | ORF4 (85%) | StrR-like transcriptional regulator (key pathway-specific regulator) |
knm8 | pdhsec (80%) | ORF5 (79%) | Pdh (prephenate dehydrogenase, tyrosine supply) |
knm9 | tba (85%) | ORF6 (84%) | ABC-transporter (GPA export) |
knmA | bpsA | ORF7 | NRPS (modules 1-2) |
knmB | bpsB | ORF8 | NRPS (module 3) |
knmC | bpsB | ORF9 | NRPS (modules 4-5-6) |
knmD | bpsC | ORF10 | NRPS (module 7) |
knm10 | mbtH (91%) | ORF11 (92%) | MbtH (NRPS chaperone protein) |
knm11 | oxyA (83%) | ORF12 (79%) | OxyA (cross-linking oxygenase, D-O-E) |
knm12 | oxyB (85%) | ORF14 (82%) | OxyB (cross-linking oxygenase, C-O-D) |
knm13 | a | a | Dahp (3-deoxy-d-arabinoheptulosonate 7-phosphate) synthase, Tei14* orthologue (76% nucleic acid sequence identity) (tyrosine supply) |
knm14 | oxyC (84%) | ORF15 (79%) | OxyC (cross-linking oxygenase, A-B) |
knm15 | bhaA (89%) | a | Tyrosine halogenase |
knm16 | bgtfA (79%) | ORF16 (71%) | GT1-family glycosyltransferase |
knm17 | bgtfB (80%) | ORF17 (77%) | GT1-family glycosyltransferase |
knm18 | bgtfC (78%) | ORF17 (75%) | GT1-family glycosyltransferase |
knm19 | orf2 (75%) | ORF21 (74%) | N-acetylglucosaminyl deacetylase |
knm20 | a | ORF22 (82%) | GT39-family glycosyltransferase (mannosyltransferase) |
knm21 | pgat (87%) | ORF24 (85%) | HpgT (4-hydroxyphenylglycine transaminase, Hpg/Dpg biosynthesis) |
knm22 | bhp (88%) | ORF25 (83%) | Thioesterase (Bht biosynthesis) |
knm23 | bpsD (86%) | ORF26 (84%) | Single-modular NRPS (Bht biosynthesis) |
knm24 | oxyD (87%) | ORF27 (84%) | Monooxygenase (Bht biosynthesis) |
knm25 | hmaS (84%) | ORF28 (84%) | HmaS (hydroxymandelate synthase, Hpg biosynthesis) |
knm26 | hmo (86%) | ORF29 (83%) | Hmo (hydroxymandelate oxidase, Hpg biosynthesis) |
knm27 | orf7 (85%) | a | Na+-H+ antiporter |
knm28 | dvaA (89%) | ORF30 (88%) | dTDP-4-dehydro-6-deoxy-α-d-glucopyranose 2,3-dehydratase |
knm29 | dvaE* | ORF31 (88%) | C4-ketoreductase |
knm30 | dvaB (90%) | ORF32 (88%) | dTDP-3-amino-2,3,6-trideoxy- 4-keto-d-glucose transaminase |
knm31 | dvaD (87%) | ORF33 (86%) | C5-epimerase |
knm32 | a | a | glucose-1-phosphate thymidylylransferase |
knm33 | dpgA (88%) | ORF35 (87%) | DpgA (type III polyketide synthase) |
knm34 | dpgB (84%) | ORF36 (79%) | DpgB (enoyl-CoA hydratase) |
knm35 | dpgC (87%) | ORF37 (85%) | DpgC (3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase) |
knm36 | a | a | Uncharacterized protein conserved in bacteria (DUF2336) |
knm37 | a | a | Porcine testicular carbonyl reductase (PTCR)-like |
