Fig. 4: (A-F) Spinning disc confocal microscopy images of N2a cells transfected with β2AR-P.D.-mCh., arrestin-3-177-mEGFP and wild-type GRK2.
From: A small molecule enhances arrestin-3 binding to the β2-adrenergic receptor
A Taken from mCh. channel after 30 s Iso stimulation. B Taken from EGFP channel after 30 s Iso stimulation. C Overlay of A and B. D Taken from mCh. channel after 5 min Iso stimulation E Taken from EGFP channel After 5 min Iso stimulation. F Overlay of (D) and (E). (G–L) Spinning disc confocal microscopy images of N2a cells transfected with β2AR- mCh., arrestin-3-177 mEGFP, and wild-type GRK2. Spinning disc confocal microscopy images of N2a cells transfected with β2AR- mCh., arrestin-3-177 mEGFP, and GRK2. G Taken from mCh. channel after 30 s Iso stimulation. H Taken from EGFP channel after 30 s Iso stimulation. I Overlay of (G) and (H). J Taken from mCh. channel after 5 min Iso stimulation K Taken from EGFP channel After 5 min Iso stimulation. Yellow arrows show arrestin-3 colocalizations with β2AR. L Overlay of J and K. Magnification 63X, scale bars are 10 µm.
