Fig. 5: FRET levels in N2a cells measured under different stimulation conditions.
From: A small molecule enhances arrestin-3 binding to the β2-adrenergic receptor
Upper left panel: Basal FRET levels in N2a cells expressing phosphorylation-deficient β2AR-mCherry, arrestin-3-177-mEGFP, GRK2, or β2AR-mCherry, arrestin-3-177-mEGFP, GRK2. Upper right panel: Calculated FRET levels in N2a cell before and after 20 µM Iso stimulation. Means ± s.e.m. are shown. **p < 0005, ****p < 0.0001 statistically significant differences. ns: no significant differences. Lower left panel: LSH-3 chemical (1H-Indole-3-acetic acid, 6-[[(6-carboxy-3-cyclohexen-1-yl) carbonyl] amino] - α- (4-ethyl- 1- piperazinyl) increases FRET between b2AR phosphorylation-deficient–mCh., and arrestin-3-177-mEGFP. Each chemical was tested in two separate experiments n = 6. Means ± s.e.m. are shown. **p < 0005, ***p < 0001, statistically significant differences. ns: no significant differences. Lower right panel: Chemical interaction with membrane targeting sequence fused mCh. and mEGFP transfected N2a cells. Means ± s.e.m. are shown. ***p < 0.001, statistically significant differences, ns: no significant differences. Chemical-treated samples were compared with Gap 43 vehicle (1% DMSO).
