Fig. 1: Consensus toxin design.

A Alignment of the long-chain α-neurotoxins included in the consensus sequence design. The alignment was performed with ClustalW using a BLOSUM62 substitution matrix, and a percentage identity matrix is detailed in Supplementary Fig. S1. The chemical characteristics of the amino acids are colour coded as shown in the legend. The final consensus sequence (LCC) is displayed. B AlphaFold2 structure prediction of LCC coloured by pLDDT scores (overall pLDDT 0.86). Numbering of loops is labelled. C Sequence conservation of the LCC based on sequence alignment to the native toxins (PDB IDs: 4FLT³⁵ and 8D9Z¹⁷). Remaining structures are AlphaFold2 predictions obtained from UniProt. D Structure conservation based on the structural alignment of native structures (grey; same structures as in C) to LCC’s structure (cyan) and corresponding RMSD values (mean RMSD = 1.8 Å).