Fig. 3: Monoclonal DELFIA screens against natural long-chain α-neurotoxins and CDR-unique clones. | Communications Chemistry

Fig. 3: Monoclonal DELFIA screens against natural long-chain α-neurotoxins and CDR-unique clones.

From: Structural mechanisms behind the neutralisation of long-chain α-neurotoxins by broadly neutralising VHHs discovered using a consensus antigen

Fig. 3

A The binding of 88 VHH clones from TPL1158 was assessed against LCC, α-cobratoxin, and two venom fractions enriched in long-chain α-neurotoxins (fractions Dv4 and Nn8). The negative control included no toxin to ensure the Eu-labelled streptavidin did not bind to the VHH clones. B DELFIA results depicting the binding of the selected 21 VHHs from TPL1158 to the various toxins. C Sequence alignment of the complementarity-determining regions (CDRs) from the selected 21 clones. The clones chosen for kinetics experiments are highlighted in bold, and their full sequences can be found in Supplementary Data 1.

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