Fig. 5: “Active Site Cap” region of KS domain contributed to the extender unit specificity. | Communications Chemistry

Fig. 5: “Active Site Cap” region of KS domain contributed to the extender unit specificity.

From: Gene conversion-associated successive engineering of modular polyketide synthases

Fig. 5

a Structural models of CmmD2-KS5 and MgmD2-KS5 created by using AlphaFold 2.0. The catalytic essential residues are colored in yellow with the indication of residues 230; (b) Sequence comparison of “Active Site Cap” region of CmmD2-KS5 and MgmD2-KS5; (c) HPLC chromatograms of engineered strains with the same treatments and amounts of fermentation broth at λ  =  280 nm with three independent repeats.

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