Fig. 1: Identification of sa-mRNA lipid adduction behavior from IL. | Communications Chemistry

Fig. 1: Identification of sa-mRNA lipid adduction behavior from IL.

From: Spotlighting the criticality of lipid quality control through a mechanistic investigation of mRNA activity loss in lipid nanoparticles

Fig. 1

a Ion Pairing-Reverse Phase (IP-RP) liquid chromatography was used to identify unadducted main peak and late eluting adducted sa-mRNA peaks using a UV detector @260 nm from a mixture of IL and sa-mRNA incubated 24 h at ambient temperature. b Analysis of LNP stability at 2–8 °C, 20 °C, and –80 °C shows mRNA lipid adduction increases over the period of 9 months. c sa-mRNA was mixed with either IL, cholesterol, a phospholipid, a PEG lipid, or with a combination of IL with one or all other lipids and lipid adduction was only observed in IL-containing mixtures. d Two lots of IL showed differential lipid adduction over a 48 h time course. e Stacked MS analysis of the two IL lots showed greater abundance of an early eluting species in lot 2, which produced more adducted mRNA.

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