Fig. 2: Mass spectrometry analysis of IL ionizable lipid and the relationship with lipid adduction.

a Stacked chromatograms using a UV detector @214 nm for analysis of four lots of IL showing various species. The four lots were used in sa-mRNA mixtures, and the extracted mRNA Late Peak (lipid adduction) correspondence with b + 32 Da, c + 14 Da, or d + 16 Da oxidation species in each lot.