Fig. 5: Identification of lipid modifications in an mRNA oligomer. | Communications Chemistry

Fig. 5: Identification of lipid modifications in an mRNA oligomer.

From: Spotlighting the criticality of lipid quality control through a mechanistic investigation of mRNA activity loss in lipid nanoparticles

Fig. 5

a IP-RP mRNA lipid adduction analysis of IL and variants IL-C and IL-F from sa-mRNA mixtures showing that only IL and IL-C containing dialkene groups form adducted mRNA. b Representative UV chromatogram @260 nm from LC-MS analysis of mRNA oligo incubated with ionizable lipid for 4 days at ambient temperature, showing two late eluting regions for peaks with two clusters of delta mass ranges. c Tables of the theoretical mass of tail and body modifications based on possible aldehyde products of peroxide degradation, which match observed delta masses with high accuracy and low mass error.

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