Extended Data Fig. 7: Non-targeted high-mass-accuracy lipidomics screen identifies significantly altered sphingolipid species in hyl-2(gnv1) mutant animals.
From: 1-Deoxydihydroceramide causes anoxic death by impairing chaperonin-mediated protein folding
Columns are as follows: measured mass per charge ratios, as detected with the LTQ orbitrap mass spectrometer and identified with in-house software; relative change between WT(N2) and hyl-2(gnv1) (increased species have red borders, and decreased species have blue borders); previously detected sphingolipid changes from Menuz et al.13 (colours as in the previous column); statistical significance as determined by unpaired two-sided Student’s t-test; FDR-corrected q-values; assigned lipid species with mass error below 2 ppm (most significantly increased assigned species are highlighted in red, and most significantly decreased assigned species are in blue); molecular formula of identified lipid species; theoretical mass per charge ratios; mass errors between measured and theoretical mass per charge ratio in parts per million. n = 6 (wild type) and 3 (hyl-2) independent biological replicates. Hex, hexose. Short hand for total back-bone carbons was used: AA:b, where AA is the total ceramide backbone carbons, and b is the number of double bonds. -OH, number of additional hydroxylations. Short hand for detailed species description was used: ihMM:n/X:Y:Z where I is iso-branched, h is sphingoid base hydroxyl groups (m, one; d, two; t, three), MM is the number of sphingoid base carbons, n is the number of sphingoid base double bonds, X is the number of fatty-acid carbons, Y is the number of fatty-acid double bonds and Z is the number of fatty acid hydroxylations.
