Extended Data Fig. 3: Rab24 reduction led to an increase in mitochondrial area.
From: Hepatic Rab24 controls blood glucose homeostasis via improving mitochondrial plasticity
Electron micrographs of mitochondria in primary hepatocytes of control (a, c) and Rab24KD (b, d) cells; higher magnifications shown in c and d. The images are representative of 12 independent wells of a 24-well plate. Scale bar 5 µm. Zoom scale bar 500 nm. Quantification of mitochondria morphology by morpho-EM for mitochondria density (e), surface area (f), perimeter or contour (g), form factor or complexity (h) and circularity (i) of control and Rab24KD cells. N=7 (CTR) and N=12 (KD) cells quantified in (e). N=329 (CTR) and N=453 (KD) in (f), N=108 (CTR) and N=(209) in (g, h, i) mitochondria analyzed. All measured after 3 days of RNAi (40 nM) in primary hepatocytes (mean +/- SEM). *P<0.05, ***P<0.001, by two-tailed unpaired Student’s t-test.
