Extended Data Fig. 7: Neuron-specific virogenetic deletion of IDOL from individual hypothalamic nuclei is insufficient to protect against diet-induced obesity.
From: IDOL regulates systemic energy balance through control of neuronal VLDLR expression
a–c, Adeno-associated virus (AAV) expressing either GFP-Cre or GFP regulated by the Synapsin I (SynI) promoter were injected into the arcuate nucleus (ARC: panels a-c) or the paraventricular nucleus of the hypothalamus (PVH: panels d-f). a–c, Deletion of IDOL from neurons in the ARC had no effect on body mass or food intake for mice fed a western diet for 12 weeks; n = 8 mice injected with AAV-expressing GFP (GFPARC-SynI), n = 8 mice injected with AAV expressing Cre-GFP (CreARC-SynI). a, An image of the ARC showing GFP-positive cells to demonstrate successful infection of neurons; the image is representative of the sixteen mice injected in the ARC study. b, Growth curve showing the mean mass of the mice from each treatment group +/− one standard deviation. c, The mean cumulative mass of food consumed per mouse +/− one standard deviation. d–f, Deletion of IDOL from neurons in the PVH had no effect on body mass or food intake for mice fed a western diet for 12 weeks; n = 8 mice injected with AAV expressing GFP (GFPPVH-SynI), n = 9 mice injected with AAV expressing Cre-GFP (CrePVH-SynI). d, An image of the PVH showing GFP-positive cells to demonstrate successful infection of neurons; the image is representative of the seventeen mice injected in the PVH experiment. e, Growth curve showing the mean mass of the mice from each treatment group +/− one standard deviation. f, The mean cumulative mass of food consumed per mouse +/− one standard deviation.
