Extended Data Fig. 1: Metabolic phenotype of IDOL-deficient mice.
From: IDOL regulates systemic energy balance through control of neuronal VLDLR expression
a,b, 18-month-old IDOL KO mice are protected against age-induced adiposity. a, Mean mass and standard error of the mean; n = 9 WT, n = 10 KO mice, ***p < 0.001, ****p < 0.0001 vs. WT by two-tailed t-test. b, Mean body fat percentage +/− the standard error of the mean; n = 9 WT, n = 10 KO mice, ***p < 0.001, ****p < 0.0001 vs. WT 2-tailed t-test. c, Growth curve for littermate male mice fed a 60% kcal high fat diet (HFD) starting when the mice were 6 weeks old. The mean values are shown +/− the standard error of the mean; n = 5 WT and n = 6 KO mice *p < 0.05 by repeat measures ANOVA. d, Growth curve for littermate male IDOL(AZ)f/f mice with or without whole-body Rosa26 Cre fed a low-fat diet from 8 weeks of age. The mean mass is shown +/− SEM; n = 10 mice per group. e, Growth curve for littermate male IDOL(AZ)f/f mice with or without whole-body Rosa26 Cre fed a high fat high cholesterol diet from 8-weeks of age; n = 8 Idol(AZ)f/f mice n = 9 CreR26+Idol(AZ)f/f mice; *p < 0.05 by repeat measures ANOVA f, Unchanged lean body mass in male IDOL knockout mice administered test diets despite adiposity changes measured by MRI. Mean lean mass is shown +/− SEM; n = 9 WT and n = 10 KO mice fed Chow, n = 5 WT and n = 7 mice fed western diet, n = 5 WT and n = 6 KO mice fed the 60% HFD, p-values calculated by two-way ANOVA with Sidak post hoc tests. g, Body length measured from nose to anus at 20 weeks of age for male mice fed either a low-fat diet (LFD) or a high-fat high-cholesterol diet (HFHC) for 12 weeks. The mean values are shown +/− the standard error of the mean. Low-fat diet: n = 10 mice per genotype. High Fat High Cholesterol diet: n = 8 Idol(AZ)f/f mice and n = 9 CreR26+Idol(AZ)f/f mice. P-values calculated by repeat measures ANOVA. h, Mean body temperature +/− SEM measured rectally in response to fasting in mice fed a high-fat high-cholesterol diet; n = 8 WT and n = 9 IDOL KO mice. The precise n-number, p-value, and details of all statistical testing are provided in the source data file.
