Extended Data Fig. 2: Adipocyte-specific APP-overexpressing mice are more sensitive to diet-induced obesity (related to Fig. 2c,d,g).
a, Representative western blotting image for APP protein levels in brain samples from control and APP transgenic mice under HFD/Dox feeding. n = 3 mice per group. b, Western blotting for APP in mitochondrial and post-mitochondrial fractions from sWAT in control and APP-overexpressing mice fed with Dox for 1 week. COXIV, mitochondrial marker; β-tubulin, cytoplasmic marker. n = 2 mice per group. Representative image chosen from three independent experiments in a and b. c, Immunofluorescence staining for APP (red), TIM23 (green) and DAPI (blue) for nuclear labelling in HEK293T cells transfected with empty vector (left) or the mito-APP construct (right). Orange colours indicate the merge between APP and TIM23. Scale bars, 20 μm. The staining experiments have been replicated three times. d, Insulin levels measured in serum samples obtained from OGTT experiments. n = 8 mice per group. Data are shown as mean ± s.e.m. of biologically independent samples. Two-way ANOVA followed by a Tukey post-test (d).
