Extended Data Fig. 4: Somatostatin secretion in the presence of glucose, dapagliflozin and CYN154806.

a, Glucagon secretion in the presence of 1 mM glucose, dapagliflozin and CYN154806 as indicated (n=6, n=9 experiments/3 mice). Glucagon secretion has been normalized to responses at 1 mM glucose. b, Somatostatin secretion in the presence of glucose, dapagliflozin and CYN154806 as indicated (n=6, n=9 experiments/3 mice). Secretion has been normalized to responses at 1 mM glucose. Statistical significances were determined by one-way ANOVA followed by Tukey’s post hoc. ***p<0.001 vs 1 mM glucose; †††p<0.001 vs 20mM; ‡‡p<0.01 20 mM glucose, dapagliflozin and CYN154806 vs 20 mM glucose and CYN154806 alone. c, Effects of dapagliflozin on delta-cell electrical activity recorded from delta cells in intact islets exposed to 20 mM glucose. In 2 out of 4 cells, dapagliflozin repolarized the δ-cell and suppressed electrical activity whereas it was without effect in the remaining 2 cells (islets from 4 different mice). d, Failure of 19 mM αMDG to evoke electrical activity in delta cells exposed to 1 mM glucose. Measurements are representative of 7 different cells in islets from 4 different mice. e, Sodium Green fluorescence (heatmap) measured in 40 different delta cells from 4 different mice simultaneously. Each line represents an individual cell (cell number indicated for the first 7 cells to the left). Note that αMDG has an effect in ~40% of the cells. All data are represented as mean ± SEM.