Extended Data Fig. 6: Long term KD feeding causes obesity in mice.
From: Ketogenesis activates metabolically protective γδ T cells in visceral adipose tissue
(a) Body weight, (b) lean mass, (c) fat mass, and (d) liver mass were measured in WT mice fed chow (n=8) or KD (n=9) for 4 months. For (a-d) Statistical differences were calculated by unpaired 2-tailed t-tests. Data are representative of 3 independent experiments. (e) Representative H&E-stained liver sections after long-term KD. Sections are representative of 2 independent experiments each with n=5 mice/group. (f) Western blot of liver AKT phosphorylation after insulin injection into fasted mice. Each lane represents an individual mouse. In KD group mice are ordered from greatest to smallest body weight (ranging from 59-37g). (g) Profile of visceral adipose hematopoietic compartment after 4 months chow (n=8) vs KD (n=8) feeding. Statistical differences were calculated by 2-way ANOVA with Sidak’s correction for multiple comparisons. All data are expressed as mean±SEM and each symbol represents an individual mouse. Exact p-values are shown whenever possible, ****p<0.0001.
