Extended Data Fig. 7: Metabolic profiling of Tcrd-/- mice after long-term KD feeding.
From: Ketogenesis activates metabolically protective γδ T cells in visceral adipose tissue
(a) Lean mass and (b) body fat in WT (n=10) vs Tcrd-/- (n=11) mice after 4 months KD. Statistical differences were calculated by nonparametric Mann-Whitney 2-tailed test because the Tcrd-/- data are not normally distributed. Data are representative of 3 independent experiments. (c) Insulin tolerance test of WT (n=10) vs Tcrd-/- (n=7) mice after 3.5 months KD. Statistical differences were calculated by paired 2-way ANOVA. (d) Western blot of liver AKT phosphorylation after insulin injection into fasted mice. Each lane represents an individual mouse. (e) Total CD45+ cellularity in Efat from WT (n=11) vs Tcrd-/- (n=11) KD-fed mice. Statistical differences were determined by unpaired 2-tailed t-test. (f) CD45+ composition in epidydimal adipose tissue in WT (n=11) vs Tcrd-/- (n=11) mice after 4 months KD. Data are pooled from 2 independent experiments with a total of n=11 WT and n=11 Tcrd-/- mice analyzed. Statistical differences were calculated by 2-way ANOVA with Sidak’s correction for multiple comparisons. Each symbol represents an individual mouse and data are represented as mean±SEM. Exact p-values are shown whenever possible, ****p<0.0001.
