Extended Data Fig. 9: Comparison of bulk sorted γδ T cell RNAseq with scRNAseq dataset.
From: Ketogenesis activates metabolically protective γδ T cells in visceral adipose tissue
(a) tSNE plot of tissue-resident immune cells from chow- and KD-fed samples shown separately to demonstrate macrophage-specific genes differentially expressed in multiple lymphoid clusters as a result of macrophage frequency change (b) tSNE plot with overlaid color that represents expression of down-regulated genes identified from bulk RNAseq data if filtered only by padj. (c) tSNE plot with overlaid color that represents expression of down-regulated (left panel) and up-regulated (right panel) genes identified from bulk RNAseq data when filtered by padj and log2FC. For all plots (a-c) data are derived from a single technical sample generated by pooling n=3 independent samples prior to sequencing for each diet group.
