Extended Data Fig. 2: Further identification of tissue-resident populations.
From: Ketogenesis activates metabolically protective γδ T cells in visceral adipose tissue
(a) tSNE plot of tissue-resident immune cells from chow- and KD-fed samples merged displaying expression of selected genes. (b) tSNE plot as in S2A showing average z-scores of genes in cell cycle pathway (Reactome database). (c) tSNE plot of proliferating cells only (cluster 10). Expression of selected markers is displayed. For (a-c) expression is based on pooled data from chow and KD samples (each containing n=3 pooled biological samples into 1 technical sample for each diet). (d) Violin plots of Il1b expression within all cells from each cluster. Expression is pooled from chow (n=4 pooled biological samples into 1 technical sample) and KD (n=3 pooled biological samples into 1 technical sample) and total number of cells in each cluster is indicated on the figure. Overlaid box plots indicate median and 25th-75th percentiles; whiskers extend no further than 1.5xIQR from either upper or lower hinge, as described in Materials and Methods.
