Extended Data Fig. 3: pHc does not globally affect cellular metabolism.
From: Cytosolic pH regulates proliferation and tumour growth by promoting expression of cyclin D1
(a–c) RPE-1 cells were starved and the relative abundance of metabolites was determined 4 hours after stimulation with the indicated conditions as described in Materials and Methods. Selected metabolites from energy (a) and central carbon metabolism (b) are shown as mean ± S.E.M.; N=4. P-values for all pairwise comparisons between conditions are listed in the Supplementary Table S7. (c) Unsupervised clustering and (d) PCA analysis were calculated using MATLAB (The MathWorks) from metabolite datasets generated using Progenesis QI software (Nonlinear Dynamics) to demonstrate the highly similar metabolic response upon stimulation in the presence or absence of DMA.
