Extended Data Fig. 7: Ablation of AgRP neurons impairs metabolic adaptation to ABA paradigm.
From: AgRP neurons control compulsive exercise and survival in an activity-based anorexia model
All studies were conducted in control and AgRPDTR mice (left) or control and AgRPTRPV1 mice (P36-P50) (right) .Sedentary mice (named S-AgRPDTR, S-AgRPTRPV1 and S-control respectively) underwent food restriction without access to the running wheel. Only Data for female mice are shown. Data are expressed as mean ± s.e.m. R-AgRPDTR n=16, S- AgRPDTR =14, R-control n=16, S-control=12. R-AgRPTRPV1 n=11, S- AgRPTRPV1=13, R-control=12, S-control n=12. Whiskers box goes from the smallest values to the largest and extends from the 25th to 75th percentiles. a, Serum Free Fatty Acid (FFA) (μM in serum) R-AgRPDTR vs all other groups **** P< 0.0001 (24 hr, 48 hr and 72 hr food restriction). AgRPTRPV1 mice, 24 hr food restriction: R-control vs S-AgRPTRPV1 * P=0.0426. 48 hr food restriction; R-control vs R-AgRPTRPV1; * P=0.0225, S-control vs R-AgRPTRPV1. * P=0.0169; R-AgRPTRPV1 vs S-AgRPTRPV1 ****P< 0.0001. b, Serum ketone bodies (mmol/L). AgRPDTR mice on the left: 24 hr food restriction: R-control vs R-AgRPDTR ** P=0.0013, S-control vs R-AgRPDTR **** P< 0.0001; R-AgRPDTR vs. S-AgRPDTR P< 0.0001. 48 hr food restriction: R-control vs S-control * P=0.0387; R-control vs S-AgRPDTR * P=0.0175: S-control vs R-AgRPDTR *** P=0.0004; R-AgRPDTR vs S-AgRPDTR *** P=0.0001. 72 hr food restriction: R-control vs R-AgRPDTR ** P=0.0022, S-control vs S-control AgRPDTR * P=0.0307; R-AgRPDTR vs S-AgRPDTR **** P< 0.0001. AgRPTRPV1 mice (on the right) 24 hr food restriction: R-control vs S-control ** P=0.0043; R-control vs R-AgRP TRPV1 *** P=0.0004: R-control vs S-AgRP TRPV1 **** P< 0.0001. 48 hr food restriction : R-control vs R-AgRP TRPV1 **** P< 0.0001; R-control vs S-AgRP TRPV1**** P< 0.0001 ; S-control vs R-AgRP TRPV1 ** P=0.0043; S-control vs S-AgRP TRPV1**** P< 0.0001, 72 hr food restriction :R-control vs R-AgRP TRPV1 **** P< 0.0001, R-control vs S-AgRP TRPV1 **** P< 0.0001, S-control vs R-AgRP TRPV1 *** P=0.0007. c, Glucose concentrations (mg/dl). R-AgRPDTR vs R-control ** P=0.0062 (24 hr food restriction). R-AgRPDTR vs all other groups **** P< 0.0001 (48 hr and 72 hr food restriction). R-AgRPTRPV1 vs S-AgRP TRPV1: * P=0.0382 (24 hr food restriction).
