Extended Data Fig. 8: Serum concentrations of active ghrelin (pg/ml).
From: AgRP neurons control compulsive exercise and survival in an activity-based anorexia model
Active ghrelin (pg/ml) was measured in serum by Elisa, Data are expressed as mean ± s.e.m. Two-way ANOVA followed by a Tukey’s multiple comparisons test. R-AgRPDTR n=4, S- AgRPDTR =4, R-control n=4, S-control=4. R-AgRPTRPV1 n=4, S- AgRPTRPV1=4, R-control=4, S-control n=4. a, b, Ghrelin concentrations in female and male AgRPDTR- and controls. Females: in all groups 24 hr vs fed ****P< 0.0001. 48 hr vs 24 hr S-control **P=0.0021, 48 hr vs 24 hr S-AgRPDTR ***P=0.0010, R- AgRPDTR 24 hr vs 48 and 72 hr ****P< 0.0001. Males: in all groups 24 hr vs fed ****P< 0.0001. S-AgRPDTR24 hr vs 48 hr ***P=0.0009; R- AgRPDTR 24 hr vs 48 and 72 hr ****P< 0.0001. C-D) Ghrelin concentrations in female and male AgRPTRPV1- and controls . Females: in all groups 24 hr vs fed ****P< 0.0001, R-control 24 hr vs 48 and 72 hr ***P=0.0004. Male: in all groups 24 hr vs fed ****P< 0.0001, R- control 24 hr vs 48 and 72 hr ***P=0.0001. R- AgRPTRPV1 24 hr vs 48 and 72 hr ***P= 0.0001.
