Extended Data Fig. 9: High fat diet rescues normal survival and prevents ABA phenotype development in male mice.
From: AgRP neurons control compulsive exercise and survival in an activity-based anorexia model
All studies have been conducted in control and AgRPDTR male mice (left) and AgRPTRPV1 mice and control mice (P36-P50) (right). Only data for male mice are shown. Data are expressed as mean ± s.e.m. SD: standard diet. HFD: high fat diet. A: acclimation, FR: food restriction, R: recovering. R-AgRPDTR SD n=14, R-AgRPDTR HFD n=13. R-AgRPTRPV1 n=12, R-AgRPTRPV1 n=13. a, Body weight changes during ABA Two-way Anova followed by Sidak’s multiple comparisons test. Day 6: R-AgRPDTR SD vs R-AgRPDTR HFD, ***P=0.0001. Day 7: R-AgRPDTR SD vs R-AgRPDTR HFD, ***P=0.0001. b, Caloric intake during acclimation (Kcal in 24 hr). Day 1, 2 and 3: R-AgRPDTR SD vs R-AgRPDTR HFD, ***P=0.0001; R-AgRPTRPV1 SD vs R-AgRPTRPV1 HFD, ***P=0.0001, c, Caloric intake during food restriction (Kcal in 2 hr). Two-way Anova followed by Tukey’s multiple comparisons test. Day 1, 2 and 3: R-AgRPDTR SD vs R-AgRPDTR HFD, ***P=0.0001; R-AgRPTRPV1 SD vs R-AgRPTRPV1 HFD, ***P=0.0001. d, Daily running wheel activity during acclimation and food restriction. Two-way Anova followed by Sidak’s multiple comparisons test.. Day 6: R-AgRPDTR SD vs R-AgRPDTR HFD ***P=0.0001, Day 7: R-AgRPDTR SD vs R-AgRPDTR HFD, ***P=0.0001. Day 9: R-AgRPTRPV1 SD vs R-AgRPTRPV1 HFD, ***P=0.0008, Day 10: R-AgRPTRPV1 SD vs R-AgRPTRPV1 HFD, ***P=0.0001 Survival during food restriction. e, Long-rank test for comparison of survival curves: R-AgRPDTR SD vs R-AgRPDTR HFD **P=0.0093 R-control vs R-AgRPTRPV1 no statistical difference, P ≥0.9999.
