Extended Data Fig. 3: Inactivation of Zfp423 exacerbates FIPs inflammatory responses.

a, Mural-Zfp423^KO (Pdgfrb^rtTA; TRE-Cre; Zfp423^loxP/loxP) mice were generated by breeding the Pdgfrb^rtTA transgenic mice to animals expressing Cre recombinase under the control of the tetracycline-response element (TRE-Cre) and carrying floxed Zfp423 alleles (Zfp423^loxP/loxP). Littermates carrying only Pdgfrb^rtTA and Zfp423^loxP/loxP alleles (that is Cre^-) were used as the control animals (Control). The addition of doxycycline (Dox) leads to inactivation of Zfp423 in Pdgfrb-expressing cells. b, mRNA levels of Zfp423 in FIPs of Control (n=3) and Mural-Zfp423^KO (n=3) mice fed Dox-containing chow diet for 10 days. c, mRNA levels of indicated pro-inflammatory genes in indicated FIPs treated with vehicle (veh.) (n=4) or 100 ng/ml LPS (n=8) for 2 hours. d, mRNA levels of genes associated with macrophage activation in cultured BMDMs following exposure to indicated FIPs conditioned media (CM) for 1.5 hours. n=4 (for groups with vehicle treatment) or n=5 (for groups with LPS treatment) independent wells of macrophages examined per experiment. e, Macrophage migration following exposure to FIPs conditioned media (CM): cell counts of migrated macrophages following exposure to indicated CM for 3 hours. n=4 (for groups with vehicle treatment) or n=9 (for groups with LPS treatment) independent wells of macrophages examined per experiment. For panels d, e FIPs (isolated from pooled depots of 6-8 mice per genotype) were treated with vehicle or LPS (100 ng/ml) for 2 hours and then incubated in serum-free medium for an additional 24 hours to produce conditioned media. Experiments in this figure were independently repeated three times. Data in this figure are shown as the mean ± s.e.m., *p< 0.05, **p<0.01 or ***p<0.001 by two-tailed unpaired Student’s t-test (b) or two-way ANOVA (c–e). Exact p values can be found in Source Data Extended Data Figure 3.

Source data