Extended Data Fig. 8: Systemic consequences of inactivating mural cell Zfp423 in obese mice housed at thermoneutrality.
From: Perivascular mesenchymal cells control adipose-tissue macrophage accrual in obesity
a, Western blot of phosphorylated AKT (pAKT), total AKT, and β-actin protein expression in tissue extracts of gonadal WAT from Control (n=3) and Mural-Zfp423KO (n=3) mice after 5-month HFD feeding. Tissues were collected before (- insulin) and after insulin stimulation (+ insulin) of anesthetized animals. b, Intraperitoneal glucose tolerance tests (GTT) of thermoneutral-housed Control (n=12) and Mural-Zfp423KO (n=16) mice after HFD feeding. * denotes p< 0.05, ** denotes p< 0.01 by unpaired two-tailed Student’s t-test. c, Intraperitoneal insulin tolerance tests (ITT) of thermoneutral-housed Control (n=8) and Mural-Zfp423KO (n=14) mice after HFD feeding. * denotes p< 0.05 by unpaired two-tailed Student’s t-test. d, Serum levels of ADIPONECTIN (left) and INSULIN (six-hour fasting) (right) in Control and Mural-Zfp423KO mice maintained on doxycycline-containing chow diet or doxycycline-containing HFD for 1 month or 5 months. For control, n=5 of chow, n=8 of 1-month HFD, n=8 of 5-month HFD; for Mural-Zfp423KO, n=6 of chow, n=11 of 1-month HFD, n=9 of 5-month HFD. e, Serum levels of indicated pro-inflammatory cytokines in Control (n=8) and Mural-Zfp423KO (n=8) mice maintained on doxycycline-containing HFD for 5 months. f, mRNA levels of pro-inflammatory genes in skeletal muscle of Control (n=8) and Mural-Zfp423KO (n=6) mice after 5-months HFD feeding. g, mRNA levels of pro-inflammatory genes in livers of Control (n=8) and Mural-Zfp423KO (n=9) mice after 5-months HFD feeding. h–j, Levels of total monocytes and pro-inflammatory monocytes (LY6C+) in blood (h), bone marrow (i), and spleen (j) of Control (n=6) and Mural-Zfp423KO (n=7) mice after 5 months of HFD feeding. Bars represent mean ± s.e.m. * denotes p< 0.05 by unpaired two-tailed Student’s t-test (d, e, g). Exact p values, numbers of repetitions, and uncropped western blots can be found in Source Data Extended Data Figure 8.
