Extended Data Fig. 9: Modulating Zfp423 expression impacts the activation of NFκB signaling in FIPs.
From: Perivascular mesenchymal cells control adipose-tissue macrophage accrual in obesity
a, Mural-Ikk2CA/Zfp423TG (PdgfrbrtTA; TRE-Cre; TRE-Zfp423; Rosa26RIkk2CA) mice were generated by breeding the Rosa26RIkk2CA allele into the Mural-Zfp423TG background. The addition of doxycycline (Dox) leads to overexpression of Zfp423 and simultaneous activation of constitutively active (CA) IKK2 in Pdgfrb-expressing cells. b, mRNA levels of Zfp423 in cultured FIPs from Control (n=4), Mural-Ikk2CA (n=4), and Mural-Ikk2CA/Zfp423TG (n=4) mice following exposure to doxycycline (1 μg/ml) in vitro for 1 day. c, mRNA levels of indicated pro-inflammatory genes in cultured FIPs from Control (n=4), Mural-Ikk2CA (n=4), and Mural-Ikk2CA/Zfp423TG (n=4) mice following exposure to doxycycline (1 μg/ml) in vitro for 1 day. d, Mural-Ikk2CA/Zfp423KO (PdgfrbrtTA; TRE-Cre; Zfp423loxP/loxP; Rosa26RIkk2CA) mice were generated by breeding the Rosa26RIkk2CA allele into the Mural-Zfp423KO background. The addition of doxycycline (Dox) leads to inactivation of Zfp423 and simultaneous activation of constitutively-active (CA) IKK2 in Pdgfrb-expressing cells. e, mRNA levels of Zfp423 in cultured FIPs from Control (n=4), Mural-Ikk2CA (n=4), and Mural-Ikk2CA/Zfp423KO (n=4) mice following exposure to doxycycline (1 μg/ml) in vitro for 1 day. f, mRNA levels of indicated pro-inflammatory genes in cultured FIPs from Control (n=4), Mural-Ikk2CA (n=4), and Mural-Ikk2CA/Zfp423KO (n=4) mice following exposure to doxycycline (1 μg/ml) in vitro for 1 day. Experiments in this figure were independently repeated three times. Data in this figure are shown as the mean ± s.e.m., *p< 0.05 or ***p<0.001 by one-way ANOVA. Exact p values can be found in Source Data Extended Data Figure 9.
