Extended Data Fig. 2: c-Rel regulates epithelial dedifferentiation and fibrogenic gene expression.
From: c-Rel orchestrates energy-dependent epithelial and macrophage reprogramming in fibrosis
a, Representative images of c-Rel staining in CCl4 injured Relflfl and RelΔHep mice. Representative of n=7 mice/group. Scale bar represents 100 μm. b, Graph shows mRNA levels of inflammatory cytokines Tnfa, Il-1b (p=0.00038) and Il6 (p value = 0.022)and the inflammatory chemokines Cxcl1 (p value = 0.034), Cxcl2 (p=0.054) and Ccl2 (p=0.029), in CCl4 injured livers of Relfl/fl and RelΔHep mice. Data are mean ± s.e.m of 7 mice/group. c, Heatmap showing relative mRNA expression of Il-6, Cxcl1, Cxcl2, Ccl2, Ccl4 and Ccl5 in WT and Rel−/− proximal tubular epithelial cells (PTEC) stimulated with or without TGFβ1. d, Graphs show relative levels of Ctgf (p value = 0.034), Ctsd (p= 0.039), Serpine1 (p=0.04) and Bmp1 (p=0.0054) protein expressed as Intensity in control and TGFβ1 treated WT and Rel-/- hepatocytes. Data are from 4 independent cell isolations/group. e, Quantification of soluble collagen (pg/ml) released from precision cut liver slices (PCLS) generated from WT and Rel-/- liver, stimulated ± TGFβ1 ± CTGF where (control p value = 0.012 and TGFβ1 p value = 0.045). Data are from PCLS generated 3 different donors/genotype. b, d, P values were calculated using unpaired two-sided t-test. (d) P values were calculated using the R package LIMMA (* P <0.05, ** P <0.01 and ***P<0.001).
