Extended Data Fig. 4: c-Rel dependent Snail regulation in chronic liver and kidney disease.
From: c-Rel orchestrates energy-dependent epithelial and macrophage reprogramming in fibrosis
a, Representative images show expression of Snail in normal and chronic CCl4 injured liver. b, Representative images show Snail in normal and UUO injured kidney. c, Representative images of Snail transcript in epithelial cells detected by RNAScope in fibrotic chronic CCl4 injured fibrotic mouse liver. d, Representative images of Snail transcript in epithelial cells detected by RNAScope in fibrotic UUO mouse kidney. All representative images are representative of n=5 mice/group. a, b, Scale bars equal 100 microns (c, d) Scale bars equal 50 microns. e, Graph showing mRNA levels of Snail in TGFβ1 treated hepatocytes (p=0.0001) and proximal tubular epithelial cells (PTEC) (p=0.02) isolated from WT and Rel-/- mice. Data in graphs are mean ± s.e.m. in n=3 independent cell isolations. f, Graph showing ChIP analysis of c-Rel binding to distal (p=0.0074) and proximal (p=0.0063) regions of the Snail promoter in WT hepatocytes stimulated ± TGFβ1. Data in graphs are mean ± s.e.m. in n=5 independent cell isolations. P values were calculated using unpaired two-sided t-test (e) and a ratio paired t-test (f) (* P <0.05, ** P <0.01 and *** P <0.001).
