Extended Data Fig. 7: Impaired lipid buffering of Marco^-/- mice is not associated with adiposity differences and weights of animals in Fig. 8d,e.

a, Weight of mice in Fig. 6c. b, Fat depot distribution of lean Marco^+/+ and Marcoⁿ mice presented as percent of bodyweight (n = 4 mice per genotype). RP: retroperitoneal, eWAT: epididymal, VAT: visceral, SAT: subcutaneous, white adipose tissue depots. c, Marco transcript levels measured in macrophages (n = 5 samples) and adipocytes (n = 4 samples). d, NEFA and glycerol levels of adipose tissue isolated from lean Marco^+/+ and Marco^-/- mice, stimulated with isoproterenol (ISO) for 2 hours (n = 5 animals; **P = 0.0022, **P = 0.0099, ****P < 0.0001; one-way ANOVA, Bonferroni’s correction). e, Weight of mice in Fig. 6d. f, Fat mass and adipose depot distribution of Marco^+/+ and Marco^-/- mice post 16 weeks HFD. RP: retroperitoneal, eWAT: epididymal, VAT: visceral, SAT: subcutaneous, white adipose tissue depots (n = 11 mice per genotype). g, Weights of mice shown in Fig. 6e. h, Macrophage content and FBC206/FBC ratios of Marco^+/+ (n = 5) and Marco^-/- (n = 7) mice prior to HFD feeding. i, Corresponding weights of mice in Fig. 8d (***P = 0.0009, ****P < 0.0001; one-way ANOVA, Bonferroni’s correction). j, Corresponding weights of mice in Fig. 8e (*P = 0.0181; one-way ANOVA, Bonferroni’s correction). Data are mean ± SEM and representative of one (b, c, g, h), two (a, d), or are pooled from two (f, j), four (e) or six (i) independent experiments.

Source data