Extended Data Fig. 1: Disruptions of adipose homeostasis and metabolic stress ablate myeloid PI3K/AKT signalling. | Nature Metabolism

Extended Data Fig. 1: Disruptions of adipose homeostasis and metabolic stress ablate myeloid PI3K/AKT signalling.

From: The PI3K pathway preserves metabolic health through MARCO-dependent lipid uptake by adipose tissue macrophages

Extended Data Fig. 1

a, Serum insulin levels of animals subjected to ST-HFD (n = 4 ND, n = 5 ST-HFD animals), 16 weeks HFD (n = 5 ND, n = 4 HFD animals) or acute lipolysis (n = 6 animals per group) (***P = 0.0006; two-tailed student’s t-test). b, Percentage of viable FB (CD45+CD11b+F4/80+) macrophages and in the adipose tissue of mice post 2 or 16 weeks HFD feeding and acute lipolysis (n = 5 animals; **P = 0.0072, ****P < 0.001, **P = 0.0026; two-tailed student’s t-test). c, Corresponding p-AKT MFI of FB-ATMs shown in (b) (*P = 0.027, ***P = 0.0001, **P = 0.0029; two-tailed student’s t-test). d, p-AKT MFI of macrophages post adipocyte conditioned media (ACM) treatment for 3 h (n = 5 samples; ***P = 0.001; two-tailed student’s t-test). Data are mean ± SEM and representative of one (a) or two (b-d) independent experiments.

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