Extended Data Fig. 7: Expression of KRASG12V in BxPC3 cells does not alter the expression levels of UCP2.
a, Expression of HA-tagged KRASG12V in control and UCP2shRNA-transfected BxPC3 cells. b, c The relative expression of UCP2 in control and UCP2shRNA-transfected BxPC3 cells expressing or not expressing KRASG12V. UCP2 transcript levels were quantified by qPCR (b) and UCP2 protein levels were assayed with a UCP2-specific antiserum (c). An anti-COX4 antibody was used for norKP-2malization. COX4 normalization was carried out on the same SDS-PAGEs used to assay HA-tagged KRASG12V (a) and UCP2 (c) expression levels. Similar results were obtained in three biologically independent experiments (a and c). For transcript analyses, reverse transcribed cDNA from three biological replicates was used and three technical replicates were analysed for each biological replicate, values represent means ± SD of three independent experiments (b). BxPC3NI, cells transfected with non-inducible lentiviral plasmids. rUCP2, recombinant human UCP2.
