Extended Data Fig. 9: A schematic representation of lentiviral plasmids used to verify the dependency of mutated KRAS on UCP2 in BxPC3 cells. | Nature Metabolism

Extended Data Fig. 9: A schematic representation of lentiviral plasmids used to verify the dependency of mutated KRAS on UCP2 in BxPC3 cells.

From: KRAS-regulated glutamine metabolism requires UCP2-mediated aspartate transport to support pancreatic cancer growth

Extended Data Fig. 9

a, b, The scramble and UCP2 silencing (shRNA1) non-inducible pLKO.1 (Blast) plasmids, used as controls, showing the BamHI and KpnI restriction sites used to clone the KRASG12V expression cassette. c, d, The scramble and UCP2 silencing (shRNA1) non-inducible pLKO.1 (Blast) plasmids carrying the KRASG12V expression cassette downstream the blasticidin resistance gene (BSD). HA-tagged KRASG12V was cloned under the control of the UbC promoter and in frame with EGFP and a "self-cleaving" P2A peptide. The efficiency of transfection (about 85%) was measured by following the EGFP fluorescence whereas the presence of P2A allowed the release of the HA-tagged KRASG12V.

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