Extended Data Fig. 6: Senescence and metabolism in Pdgfrb-TERT-KO (b-KO) mice.
From: Age-associated telomere attrition in adipocyte progenitors predisposes to metabolic disease
a, Quantitative RT-PCR demonstrates higher senescence marker and pdgfa induction in VAT than in SAT of 8-month old b-KO males compared to WT males. Data normalized to 18S RNA. N = 5 mice / group. b, Comparable food intake by b-KO and WT males. c, Comparable spontaneous locomotor activity of b-KO and WT males. d, RER in males calculated based on oxygen consumption (VO2) and carbon dioxide production (VCO2). e, Indirect calorimetry-based measurement of energy expenditure in b-KO and WT males at 8 months of age. f, Comparable cold tolerance in b-KO and WT males placed at 4 °C. g, H&E staining does not reveal abnormality in BAT and liver of b-KO males at 8 months of age. Scale bar=50 µm. h, Echo MRI data: no body composition abnormality in b-KO females at 8 months of age. i, ITT: no insulin resistance in 8-month-old b-KO females. j, Comparable cold tolerance in b-KO and WT females placed at 4 °C. N = 6 mice / group. P: Student’s t-test (2-sided).
