Extended Data Fig. 1: Telomere attrition / cell senescence in WAT of a-KO mice.
From: Age-associated telomere attrition in adipocyte progenitors predisposes to metabolic disease
a, TRF assay reveals advanced telomere shortening in SAT of both a-KO males (M) and females (F) at 8 months of age. b, Telo-FISH staining (red) on sections from Pdgfra-Cre;mTmG;TERTfl/fl (WT) and Pdgfra-Cre;mTmG;TERTfl/fl male mice reveals normal telomeres in mG+ cells of brain and muscle. c, Telo-FISH staining on culture-plated cells from VAT and SAT of control Pdgfra-Cre; mTmG and Pdgfra-Cre; mTmG; TERTfl/fl mice reveals advanced telomere shortening (less red signal) in SAT mG+ cells of a-KO mice. d, Telo-FISH staining on SAT sections from mice in c reveals telomere shortening and nuclear p16 IF signal in mG+ cells of a-KO mice. e, q-PCR reveals telomere shortening in SAT of 8-month old WT mice compared to 3-month old WT mice. Evident is more advanced telomere shortening in SAT of 8-month old α-KO males (M) and females (F), compared to 8-month old WT males and females, respectively. f, Quantitative RT-PCR analysis reveals higher p16 and p21 mRNA expression in VAT and SAT of a-KO mice, compared to WT mice, at 6 months of age. Data are normalized to 18S RNA. N = 3 mice / group. Scale bar=50 µm in all panels. P: Student’s t-test (2-sided).
