Extended Data Fig. 4: Additional validations for the Z+R category.
From: Space-time logic of liver gene expression at sub-lobular scale
a-b, smFISH (Stellaris, Methods) for Elovl3 (Z+R) and Arg1 (Z+R). smFISH quantifications were made for ZT0 and ZT12 (Methods). Left: representative images at ZT0, ZT12 for Elovl3 (a) or Arg1 (b). Pericentral veins (CV) and a periportal node (PN) are marked. Scale bar - 20 µm. Right: quantified profiles for each gene at the two time points from smFISH (top, line plot is the mean number of mRNAs, shaded area indicate SD across twelve images), and scRNA-seq data (bottom, line plot is mean expression, shaded area is SD across mice, n=2 or n=3 depending on the time point, Methods).
