Extended Data Fig. 6: NRF2 expression and activity during tumour recurrence.

a, qRT-PCR analysis of Nqo1, Slc7a11, Gclm, and Hmox1 expression in primary tumour cells expressing an empty vector (Control) or caNRF2. Data are shown as the mean ± SEM for n = 3 biologically independent replicates. Significance was determined by two-way ANOVA (Tukey’s multiple comparisons test). Data are representative of 2 independent experiments. b, DCFDA staining showing ROS levels in primary tumour cells expressing an empty vector (Cont) or caNRF2. Data are shown as the mean of n = 2 biologically independent replicates and are representative of 2 independent experiments. c, Kaplan-Meier plot showing primary tumour-free survival for mice bearing control tumours (n = 27 independent tumours) or caNRF2 tumours (n = 32 independent tumours). Hazard ratio and P value were determined by two-sided log-rank (Mantel-Cox) test. d, Western blot for NRF2 and NQO1 in caNRF2-expressing primary and recurrent tumours. Blot is representative of a single experiment. e, Western blots for NRF2 and NQO1 in control primary and recurrent tumours. Blot is representative of a single experiment.