Extended Data Fig. 8: NRF2 has limited effects on metabolism in primary tumour cells.

a, Relative GSH:GSSG ratio in primary tumour cells expressing empty vector (control) or caNRF2. Data are shown as the mean ± SEM for n = 3 biologically independent replicates. Significance was determined by two sided Student’s t test. b–d, qRT-PCR analysis of Gclm and Slc7a11 (b), Txnrd1 (c), and Pgd (d) expression in control (shScr) and NRF2-knockdown (shNRF2) primary tumour cell lines. Data are shown as the mean ± SEM for n = 3 biologically independent replicates. Significance was determined by two-way ANOVA (Tukey’s multiple comparisons test). e, Relative levels of the PPP metabolites 6-phosphogluconate (6PG) and ribose 5-phosphate (R5P) in primary tumour cells expressing an empty vector (control) or caNRF2. Data are shown as the mean ± SEM for n = 3 biologically independent replicates. p values were determined by two-sided Student’s t test. f, Waterfall plot showing the fold change in nucleotide levels between primary tumour cells expressing an empty vector (control) or caNRF2. g, Relative levels of the PPP metabolites 6PG and R5P in primary tumour cells cultured with dox (Her2 on) and without dox for 7 days (Her2 off). Data are shown as the mean ± SEM for n = 3 biologically independent replicates. Significance was determined by two-sided Student’s t test.