Extended Data Fig. 4: Non-essential amino acids support proliferation of tumour cells in the absence of glutaminases.
From: Identifying strategies to target the metabolic flexibility of tumours
a, b, Total levels of NEAA measured in extracts from either CT and Gls1KO tumours (a; n=5 mice per group; GC-MS), or CT/shLuc and Gls1KO/shGls2 tumours (b; n=6 mice per group; GC-MS). c, Proliferation of cells derived from either CT, Gls1KO/shLuc or Gls1KO/shGls2 HCCMYC tumours in the indicated conditions. Representative curves from one of three independent experiments (with three biological replicates) are shown. d, Quantification of Krebs cycle metabolites and amino acids in extracts from the CT, Gls1KO/shLuc or Gls1KO/shGls2 HCCMYC cells grown in control conditions (Data represents the average of three different experiments). e, 15N-enrichment in the indicated amino acids from CT and Gls1KO/shGls2 tumours after a [15N]alanine bolus (n=4 mice per group). GC-MS. Data are presented as mean ± S.D. Statistical analysis was performed using a two-tailed Student’s t-test. Complete list of exact P values is provided as a source data file.
