Extended Data Fig. 1: NFYB-1 regulates mitochondrial function and longevity (Related to Fig. 2).
From: NFYB-1 regulates mitochondrial function and longevity via lysosomal prosaposin
a, Mini lifespan screen of WT and isp-1(qm150) upon RNAi knockdown of candidate genes from egg on in comparison to luci RNAi control bacteria (N = 1, n = 120 per condition). b, Representative image of mKate2::NFYB-1 nuclear (nuc) localization in various tissues of AL day 1 adults, scale bar = 10 μm. c, Representative immunoblot of mKate2::NFYB-1 levels over age in AL WT (day 1, 3, 5, 9 and 13 as mentioned, n = 200 worms per repeat per condition), with quantitation and histone loading control. d-e, nfyb-1(cu13) mutations leads to a decrease in pcco-1::gfp expression in AL day 1 at 25 °C. d, Representative images of ca. n = 10–20 worms, scale bar = 75 μm, e, pcco-1::gfp expression levels normalised to (Time of Flight) TOF quantitated using biosorter (n ≥ 400). f, nfyb-1(cu13) mutation results in a decrease in relative basal oxygen consumption rate (OCR) compared to WT, and in germline-less glp-4(bn2);nfyb-1(cu13) compared to glp-4(bn2) animals at 25 °C, upon ARD recovery (n = 900 worms). g, Kaplan–Meier survival curves of WT, nfyb-1(cu13) upon ARD recovery shows that nfyb-1 loss leads to decrease in survival (n = 120 per repeat, per condition). h, i, Percentage decrease in mean and median survival of nfyb-1(cu13) in comparison to WT in AL and upon ARD recovery. j, cco-1 mRNA levels are reduced in nfyb-1(cu13). k, mRNA levels of nfyb-1 mitochondrial regulated genes, l, loss of nfyb-1 does not affect mRNA levels of atfs-1, dve-1 and skn-1, j-l quantitation by qPCR at AL day 1 (n ≥ 1000). b-l, All data represent N = 3 independent biological replicates, n = total worms per condition from three replicates unless stated otherwise. Error bar shows mean± s.e.m., statistics determined by c, j, l one-way ANOVA and e, f, k two-sided t-test, ns: not significant, *P < 0.5, **P < 0.01, ***P < 0.001. a, g-i, Two-sided Mantel–Cox log-rank test, refer to Supplementary Table 2 for statistics. d-f, ARD day 10 worm recovered for 1day.
