Extended Data Fig. 3: NFYB-1 omics analyses, ER genes only weakly modulate mitochondrial longevity (Related to Fig. 3).
From: NFYB-1 regulates mitochondrial function and longevity via lysosomal prosaposin
a, Workflow of transcriptomics and proteomics analysis for indicated genotypes on AL day 1 adult worms. Heat maps represent log2-fold change of differentially expressed transcripts and proteins (P < 0.05). b, Averaged PCA (Principal Component Analysis) of most variable differentially expressed genes in transcriptomics and proteomics of day 1 adults for genotypes WT, nfyb-1(cu13), isp-1(qm150) and isp-1(qm150);nfyb-1(cu13), (Omics: transcriptomics N = 3, proteomics N ≥ 5 independent biological replicates. n ≥ 5000 worms per condition per repeat). c, Mean lifespan of isp-1(qm150) and isp-1(qm150);nfyb-1(cu13) upon RNAi knockdown of differentially expressed proteins or luciferase control RNAi (luci) (n = 120 per repeat, per condition), spp-8i shows the most prominent rescue of lifespan. d, nfyb-1(cu13) elevates expression of UPRER marker, phsp-4::gfp, which is dependent on UPRER factors ire-1 and xbp-1, day 1 adult, quantitation by biosorter (n ≥ 400). e, Kaplan–Meier survival curve of isp-1(qm150);nfyb-1(cu13) upon RNAi knockdown of UPRER factors show no significant regulation of lifespan (N = 2 independent biological replicates, n = 120 per repeat, per condition). c, d, All data represent N = 3 independent biological replicates unless stated otherwise, n = total worms per condition from three replicates unless stated otherwise. Error bar shows mean ± s.e.m, d, statistics determined by one-way ANOVA, *P < 0.5, **P < 0.01, ***P < 0.001. c, e, Two-sided Mantel–Cox log-rank test, refer Supplementary Table 2 for statistics.
