Extended Data Fig. 2: Bmp8b is overexpressed by primary hepatocytes when cultured in vitro.
From: Bone morphogenetic protein 8B promotes the progression of non-alcoholic steatohepatitis
a, Relative mRNA gene expression levels of Bmp8b measured by quantitative real-time polymerase chain reaction (RTqPCR) in PH cultured at low (20,000 cells/cm2) or high (100,000 cells/cm2) confluence to model a highly proliferative (b; Cyclin E1, Ccne1) or differentiated (c; Albumin, Alb) behavior, respectively. Bmp8b mRNA expression levels are very low at baseline, and induced from 24h after culturing. All the results are shown as mean ± s.e.m. Statistical significance was assessed by Multivariate Analysis of Variance (MANOVA; 5 replicates/group). d, Microarray data from publicly available database GSE122660 of human primary hepatocytes (PH) and human hepatocytes cell lines (HepG2 and Huh7) cultured in 2D and challenged for 72h with DMSO or a mixture of oleic (OA) and palmitic (PA) fatty acids (with/without TNFα) suggest that also human hepatocytes express BMP8B and that the challenges with fatty acids and/or pro-inflammatory factors do not influence BMP8B expression. Expression data were retrieved using the tool Geo2R from NCBI, and statistical significance was assessed using One-Way Analysis of Variance (ANOVA; 3 replicates/group).
