Extended Data Fig. 4: Recombinant BMP8 rescues the defect in HSC activation observed in Bmp8b KO HSC cultured in vitro.
From: Bone morphogenetic protein 8B promotes the progression of non-alcoholic steatohepatitis
Freshly isolated HSC from Bmp8b KO mice and WT littermates were cultured for 4 and 6 days at a density of 35K cells/cm2 in presence/absence of recombinant BMP8 (30 mg/mL) to study the effect of BMP8b gain of function/recovery in both KO and WT cells. αSMA protein expression (a,b, IF; staining repeated twice in each biological replicate; magnification: 10X), and gene expression (RTqPCR) of multiple markers of HSC transactivation (c), inflammation (d) and TGFβ/BMP targets (e) were checked to study HSC’s activation status (4 biological replicates/group; each biological replicate is a pool of three livers). All the results are shown as mean ± s.e.m. Statistical significance was assessed by Multivariate Analysis of Variance (MANOVA).
