Extended Data Fig. 6: Bmp8b KO mice challenged with CDHFD model (NASH F2 fibrosis).

BMP8b KO and wild-type littermates mice were treated for 14 weeks with a choline deficient high-fat diet (CDHFD – N: 9WT & 6KO). CDHFD -treated Bmp8b KO mice show no difference in BW (a), Liver to body weight percent ratio (b: LW/BW%), glucose and lipid metabolism (c), ALT (d), NASH activity (e, H&E; f, NASH activity score) and Fibrosis (g, Picro-Sirius Red, PSR; h, “Kleiner” Fibrosis Stage; i, PSR quantification (% of stained area quantified using HALO imaging software, Indica Lab) on the whole-tissue scanned slide; j, Procollagen C3, PRO-C3). However, the relative mRNA expression levels of key genes measured by RTqPCR in the livers (k), and in freshly isolated HSC (l; Sample size: 6WT & 3 KO HSC pools) show impaired activation of TGFβ/BMP signaling, reduced inflammation, and defective HSC activation in Bmp8b KO mice compared to WT littermates. a-l, All the results are shown as mean ± s.e.m. (biological replicates are represented as dot plots). Statistical significance was assessed by two-sided Student T-Test.