Extended Data Fig. 1: In liver disease, BMP8b is overexpressed in Albumin and αSma positive cells.
From: Bone morphogenetic protein 8B promotes the progression of non-alcoholic steatohepatitis
a, Relative mRNA expression levels of Bmp8b measured by quantitative real-time polymerase chain reaction (RTqPCR) in murine livers. The levels of BMP8b mRNA transcript increase in Western Diet (WD)-induced NASH [(CTRL) n: 8; (WD) n:8], but not in High Fat Diet (HFD)-induced NAFL [(CTRL) n:5; (HFD) n:6], and in acute [3 days; (CTRL) n: 6; (CCl4) n:4] (3 days; n:4-5/group) and chronic [8 weeks; (CTRL) n: 5; (CCl4) n:5] carbon tetrachloride (CCl4) -induced liver damage. All the results are shown as mean ± s.e.m.; expression data of biological replicates represented as dot plots; statistical significance (vs. control treatment) assessed by two-sided Student’s t-test. b-c, Representative IF of Bmp8b and Albumin (Alb) or αSma protein expression: in Western Diet (WD)-induced NASH and following acute (3 days) CCl4 challenge Bmp8b is expressed in the liver, and co-localizes with both αSma and Albumin thus suggesting that Bmp8b is expressed in hepatocytes and activated HSC following hepatic damage (2 replicates / condition; staining repeated twice).
