Fig. 5: Transformed cells display a diverse capacity to utilize inosine as a carbon source.
From: Inosine is an alternative carbon source for CD8+-T-cell function under glucose restriction
a, Growth curves for HeLa cells in the indicated conditioned media were determined by live-cell imaging analysis (IncuCyte ZOOM). Data are presented as mean ± s.d. (n = 4). ***P < 0.0001 for 2 mM inosine versus glucose free by two-way analysis of variance (ANOVA). Data are representative of three independent experiments. b, LAN-1 cells were cultured in the indicated conditioned media, and cell-growth curves were monitored and analysed by IncuCyte ZOOM. Data are presented as mean ± s.d. (n = 4). ***P < 0.0001 for 2 mM glucose versus 2 mM inosine by two-way ANOVA. Data are representative of three independent experiments. c,d, HeLa cells were incubated with [13C6]glucose or [13C5]inosine for 24 h, extracted as described in the Methods and analysed for PPP metabolites (c) and glycolytic and Krebs-cycle metabolites (d) by IC–UHR-FTMS. All symbols and abbreviations are as described in Fig. 2. Numbers on the x axes represent the numbers of 13C atoms in given metabolites. Values represent mean ± s.e.m. (n = 3). Sample size (n) represents biologically independent samples (a–d).
