Extended Data Fig. 9: Confirmation of gene knockout or overexpression after lentiviral transduction.
From: Selenium detoxification is required for cancer-cell survival
a, Immunoblots of SEPHS2 in 5 non-transformed (blue) and 5 transformed (black) cell lines transduced with Cas9/sgRNAs against SEPHS2, and control (non-targeting sgRNA). b, Immunoblots of SEPHS2 in cells transduced with CRISPR resistant SEPHS2_U60C and control (empty vector). c, Immunoblots of SLC7A11 in cells transduced with Cas9/sgRNAs targeting SLC7A11 or non-targeting sgRNA. d, Immunoblots of SLC3A2 in cells transduced as indicated e, Immunoblots of PSTK in cells transduced as indicated. f, Immunoblots of SEPHS1 in 2 non-transformed and 2 cancer cells transduced as indicated. g, Immunoblots of GPX4 in cells transduced as indicated. h, Immunoblots of FSP1 in cancer cells transduced as indicated. All samples were lysed between 7~8 days after viral transduction.
